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Cultivation Technology of Shiitake Mushroom (Lentinula edodes)

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Cultivation technology of Shiitake mushroom (Lentinula edodes)


The cultivation technology of the edible and medicinal mushroom Lentinula edodes commonly called as Shiitake mushroom has been standardized. The flow chart of the technology is as follows.


Substrate preparation

(Mixture of hard wood sawdust, wood chips, Rice bran and calcium sulphate)



Fill one Kg wet substrate (65% moisture) to Polypropylene bags, plug with non-absorbent cotton



Sterilize the substrate (121°C, 15 lb pressure for 90-120 minutes in autoclave)



Cool the bags to room temperature


Spawning (Aseptically, @ 5% of wet substrate)


Spawn running (22-26°C, 60-70 days)

Initial spawn running





Mycelial coat formation



Blister formation



Open bags for browning





Cold water shock treatment (10-15°C water, 6-8 hours)



Fructification (22-26°C, 80-85% R.H, Light, cross ventilation)


Pinheads                                 Ready to harvest mushrooms


Sold as fresh or dry mushroom


Services offered by the mushroom lab, IIHR

  1. Spawn of commercial varieties of Oyster mushroom (Pleurotus species), Milky mushroom (Calocybe indica), Shirotamogitake (Hypsizygous ulmarius) and Shiitake (Lentinula edodes) can be procured in person on prior order basis.
  2. Regular training courses are offered on Mushroom seed production and Mushroom cultivation technology separately.
  3. Literature – Mushroom cultivation Bulletin, folders on mushroom cultivation.


Further details: Director, IIHR, Hessaraghatta lake post, Hessaraghatta, Bangalore-560089, E-mail: