Chairman : Director,IIHR
Members : All Scientists of the Institute
Member-Secretary : Dr. P C TRIPATHI.
The 84th IRC Meeting was held during April to May 08th, 2014, under the Chairmanship of Director, IIHR, Bengaluru. All the ongoing research projects of the Institute were reviewed and the plan of work for the year was formulated. Many new research projects were also presented for consideration of the house. Following are the major recommendations of the IRC.
1. Evaluation of under-utilized fruits for yield, quality and adaptability:There is need for prioritizing the crops for in depth studies, accordingly, Rose-apple, Sweet Tamarind, Bael, Wood apple and Khirni were suggested as prioritized crops.
2. Incorporation of bacterial blight resistance in pomegranate: There is need to screen the sub-Himalayan collections and progenies derived from chemical and physical mutagens for bacterial nodal blight resistance.
3. Breeding tomato for resistance to biotic and abiotic stresses and gene pyramiding for ToLCV resistance:Work on GBNV may be intensified with Ty genes background.
4. Breeding okra varieties / hybrids for yield, quality & resistance to biotic stresses through marker-assisted selection (MAS): Genetics of resistance to YVMV should be worked out in resistant lines different genetic system in okra.
5. Evolving F hybrids in tropical carrots with high yield and quality through marker assisted selection (MAS): Genes and their inheritance pattern to be studied before development of molecular markers for male sterility and nematode resistance in carrot.
6. Evolving rose varieties (both open and polyhouse) for quality and resistance to powdery mildew, black spot and thrips:
- Breeding programme in rose for cut flowers for export purpose should be targeted especially FOR red and orange colours, as they are in great demand.
- Root stock studies have to be taken up considering Natal Briar as a possible replacement. A study on the life span of the crop with respect to root stocks may be taken up. This should also address the issues of abiotic stress like soil salinity and poor quality water.
7. Development of recombinant protein and phage display based diagnostic kits for citrus greening bacterium, Citrus Tristeza virus and cucumber mosaic virus:Diagnostics based on phage display to be concluded by next year.
8. Development of molecular diagnostics for rapid detection of quiescent infections of Colletotrichum and Alternaria on fruits and vegetables:
- Contribution of each pathogenic factor for yield loss in a crop needs to be addressed rather than single pathogen in targeted vegetable crops.
- Copper residues due to Bordeaux mixture application in the tomato fruits to be monitored.
9. Studies on the mechanism of the Virus-Vector host interactions in virus disease of vegetable crops: Temporal and spatial analysis of PRSV and aphid vector needs to be studied using most recent software available.
10. Integrated management for pre and postharvest diseases in fruits and vegetables: Post-harvest disease scoring to be made based on sporulation, lesion size and lesion development instead of lesion size alone.
11. Integrated disease management for bacterial blight of pomegranate and bacterial wilt of tomato and brinjal: Develop molecular probe for soil diagnostics of wilt and oily leaf spot pathogen.
12. Development of IPM for major pests of cucurbitaceous vegetables: Demonstration of cue-lure and bait spray/ splashes to manage melon flies on bitter gourd may be taken up in the current rainy season.
13. Monitoring and management of insecticide resistance horticultural crops ( Thrips, mealy bugs, mites, brinjal shoot and fruit borer etc.): Baseline susceptibility data for new chemicals used against L. orbonalis like rynaxypyr, indoxacarb, flubendiamide may be generated.
14. Biochemical and molecular assessment of chilling injury in mango: Specific biochemical markers for chilling injury may be defined from the work carried out so far under the sub-project.
15. Development of microbial consortium for sustainable production of horticultural crops and improving soil health: All microbial consortium should be advised for use with recommended levels of FYM/Compost as otherwise the effect of consortium will not be satisfactory.
16. Assessment and refinement of IIHR technologies through farmers' participatory demonstrations: While conducting demonstrations on IIHR technologies, data may be collected to identify the factors which are contributing to higher or lower yield in different farmers' field. Through this effort a checklist may be developed on factors contributing to higher yield.
17. Mapping hotspot areas of horticultural gene pool, distribution and data base development:
- Diversity maps for mainstream indigenous horticulture crops and their wild species need to be generated using ARC GIS software, identifying hotspot areas.
- Ground survey data should be corroborated with satellite generated data through a temporal periodicity.
- Introspection of the data generated by the satellite images specifying their limitations and come out with close to the reality information.
18. Optimization of germplasm domestication strategies for introducing new species of horticultural importance for crop diversification: Minor fruit, Canthium parviflorum (local name: Kare) diversity may be collected from surrounding areas.
19. Development of microsatellite markers in okra (Abelmeschous esculantus L. Moench): It is important to develop a marker-linked to YVMV and also correlate copy number to the level of resistance since okra has a variable ploidy level. Exploration for funding through a BIRAC programme in collaboration with companies like Chromus who are already into developing markers for okra may be explored.
20. Agrobacterium and Biolistic mediated transgenic development using AMP gene:
- Vector control should be used in the transformation protocol and reported genes such as GFP to validate transformation protocols.
- Tomato transgenic with AMP, defensin and Pflp may also be tested for resistance to Ralstonia and Xanthomonas.
- Establish consistent and efficient regeneration and transformation protocol using cotyledon with vectors having reporter genes.
21. Development of Bt transgenic brinjal for resistance to the shoot and fruit borer, Leucinodes orbonalis Guenee: It is important to generate molecular biology data such as PCR, southern, ELISA, quantitative ELISA, junction fragment analysis on selected events, correlate LC50 with level of protein expression and he should take it to event selection trials.
22. Ultra drying as a cost effective technique to extend seed longevity of horticultural germplasm under ambient conditions (Crops - papaya, onion, china aster): Ultra drying technique for storage of large quantity of seeds needs to be explored.
23. Value addition to seeds through coating and pelleting in horticultural crops (papaya, onion, carrot, China aster): Work on seed coating, pelleting with growth promoting bacteria and micro nutrients to enhance seed germination needs to be done.
24. Socio-economic impact of horticultural technologies on crop diversification, farm income, employment and trade: Policy Analysis Matrix (PAM) may be attempted to study the impact of policy changes on the export performance of processed fruit and vegetable products.
25. Economics of factor productivity and production efficiency in horticultural crops: Decline in factor productivity in horticulture is a serious concern. This project must bring out the factor productivity of all those inputs which are indiscriminately used leading to imbalances and decline in productivity like fertilizers, water, pesticides etc.