Developed liquid and talc based formulation of novel biocontrol agent Bacillus subtilis (IIHR BS-2, NAIMCC – B-01211). Highly effective against eggs and juveniles of root knot nematodes (Meloidogyne incognita) infecting horticultural crops with killing efficiency of up to 90.26%. Recorded yield increase of 26.5% in okra, 19.2% in tomato, 22% in banana. Shelf life 18 months; Toxicology data is generated.