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Cultivation Technology of Reishi Mushroom

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Cultivation technology of Ganoderma lucidum

The cultivation technology of the medicinal mushroom Ganoderma lucidum commonly called as Reishi mushroom has been standardized on locally available substrates. The flow chart of the technology is as follows.

Substrate preparation

(Mixture of wet sawdust and rice bran)


Fill one Kg wet substrate (65% moisture) to Polypropylene bags, plug with non-absorbent cotton



Sterilization of the substrate (121°C, 15 lb pressure for 60-90 minutes in autoclave)


Cool the bags to room temperature



Spawning (Aseptically, 5% of wet substrate)



Spawn running in dark room (28-30°C, 25-35 days)




Initial spawn running




Open the bags and shift to cropping room (28-35°C, 80-85% R.H, Light, cross ventilation)



Pinhead initiation (10-15 days after opening)




Pin heads



Fruit body expansion (kidney shaped, reddish to brown in color, 15-20 days)



Harvesting (When the white color of the growing edge just disappears)



       Mature mushrooms



Sold in various forms of dry mushroom, mushroom powder and products




Services offered by the mushroom lab, IIHR

  1. Spawn of commercial varieties of Oyster mushroom (Pleurotus species), Milky mushroom (Calocybe indica), Shirotamogitake (Hypsizygous ulmarius) and Shiitake (Lentinula edodes) can be procured in person on prior order basis.
  2. Regular training courses are offered on Mushroom seed production and Mushroom cultivation technology separately.
  3. Literature – Mushroom cultivation Bulletin, folders on mushroom cultivation.


Further details: Director, IIHR, Hessaraghatta lake post, Hessaraghatta, Bangalore-560089, E-mail: