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Cultivation Technology of Milky Mushroom

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Cultivation Technology of Milky Mushroom

The cultivation technology of Calocybe spp. commonly called as Milky mushroom has been standardized on locally available substrates. This is the first Indian mushroom variety to be commercialized by IIHR. This variety is a tropical species and has excellent shelf life. The flow chart of the technology is as follows.

Substrate preparation

(Paddy or wheat straw)



Chopped in small (1-2cm pieces), soaked in water for 2-3 hours






Substrate can be pasteurized by hot water (80°C) for 2hours (no prior soaking required in this case), suitable for small scale production OR steam pasteurization at 80°C for 2 hours (in autoclave or special steam chambers), suitable for semi-commercial production OR sterilized in an autoclave (after filling in PP bags, plugged with cotton) at 121°C, 15 lb pressure for 15 minutes (best method for commercial production)




Hot water pasteurization      steam pasteurization        sterilization   


Fill one Kg pasteurized substrate (65% moisture) in Polypropylene bags, plug with non-absorbent cotton





Spawning (Aseptically, @ 5% of wet substrate) while filling in bags in pasteurization method OR top spawning in sterilized method






Spawning in pasteurized bags         Top spawning in sterilized bags




Spawn running in dark room (30-38°C, 25-30 days)